Biotechnology: Principles and Processes MCQs Mock Test of Class 12th Biology

Welcome to your Biotechnology: Principles and Processes MCQs Mock Test of Class 12th Biology

1.

Q1. An antibiotic resistance gene in a vector usually helps in the selection of:

(i) Competent cells

(ii) Transformed cells

(iii) Recombinant cells

(iv) None of the above.

2.

Q2. DNA element with ability to change its position is called:

(i) Cistron

(ii) Transposon

(iii) Intron

(iv) Ricon

3.

Q3. The term ’chemical knife’ refers to

(i) polymerases

(ii) endonucleases

(iii) ribonucleases

(iv) cellulases.

4.

Q4. Who among the following was awarded the Nobel Prize for the development of PCR technique?

(i) Herbert Boyer

(ii) Hargobind Khorana

(iii) KaryMullis

(iv) Arthur Kornberg.

5.

Q6. Which of the following is not used to transfer the recombinant DNA into the host ?

(i) Micro-injection method

(ii) Gene gun method

(iii) Bioreactors

(iv) Disarmed pathogen vectors

6.

Q7. Which one is called molecular scissors?

(i) Endocrine

(ii) Ribonuclease

(iii) Restriction enzymes

(iv) Exonuclease

7.

Q8. Restriction’ in Restriction enzyme refers to:

(i) Cleaving of phosphodiester bond in DNA by the enzyme

(ii) Cutting of DNA at specific position only

(iii) Prevention of the multiplication of bacteriophage in bacteria

(iv) All of the above.

8.

Q9. Two microbes found tb be very useful in Genetic Engineering are:

(i) Excherichia coli and Agrobacterium tumefaciens

(ii) Vibrio cholarae and a tailed bacteriophage

(iii) Diplococcus sp. and Pseudomonas sp.

(iv) Crown gall bacterium and Caenorhabdities elegans

9.

Q10. Which of the following is required to perform polymerase chain reaction ?

(i) Primers, dNTPs and DNA polymerase

(ii) DNA, CaCl2 and nuclease

(iii) Mg + 2, DNA

(iv) Both (i) and (iii)

10.

Q11. Brief exposure of cells to an electric field also allow the bacteria to take up DNA and this process is called as

(i) Apheresis

(ii) Electroporation

(iii) Electrolysis

(iv) Electrophoresis

11.

Q12. Who discovered recombinant DNA (rDNA) technology?

(i) James D. Watson

(ii) Walter Sutton and Avery

(iii) Stanley Cohen and Herbert Boyer

(iv) Har Gobind Khorana

12.

Q13. Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

(i) DNA can be seen in visible light

(ii) DNA can be seen without staining in visible light

(iii) Ethidium bromide stained DNA can be seen in visible light

(iv) Ethidium bromide stained DNA can be seen under exposure to UV light.

13.

Q14. Who discovered Restricted endonucleas enzymes?

(i) Smith and Nathane

(ii) Baiger

(iii) Waxman

(iv) Fleming

14.

Q15. Enzyme ‘Taq polymerase’ used in PCR, has been isolated from bacterium

(i) Agrobacterium tumefaciens

(ii) Thermus aquaticus

(iii) Streptomyces albus

(iv) Escherichia coli

15.

Q16. Process used for amplification or multiplication of DNA in DNA fingerprinting is

(i) polymerase chain reaction

(ii) southern blotting

(iii) northern blotting

(iv) none of these.

16.

Q17. Restriction endonucleases are enzymes which

(i) make cuts at specific positions within the DNA molecule

(ii) recognize a specific nucleotide sequence for binding of DNA ligase

(iii) restrict the action of the enzyme DNA polymerase

(iv) remove nucleotides from the ends of the DNA molecule

17.

Q18. A recombinant DNA molecule can be produced in the absence of the following:

(i) Restriction endonuclease

(ii) DNA ligase

(iii) DNA fragments

(iv) E. coli.

18.

Q19. Plasmid is a:

(i) Fungus

(ii) Plasmid

(iii) Part of Plasma membrane

(iv) Extra chromosomal DNA in bacterial cell

19.

Q20. The extraction of DNA from the agrose gel is called as

(i) Elution

(ii) Ligation

(iii) Transformation

(iv) Isolation

20.

Q21. Helium is used in Biolistic gun as

(i) It is a light gas

(ii) It acts as a propellant

(iii) It is an inert gas

(iv) Tungsten coated with DNA can be easily passed in the presence of Helium

21.

Q22. Rising of dough is due to

(i) Multiplication of yeast

(ii) Production of CO2

(iii) Emulsification

(iv) Hydrolysis of wheat flour starch into sugars.

22.

Q23. Which of the following is not used to transfer the recombinant DNA into the host ?

(i) Micro-injection method

(ii) Gene gun method

(iii) Bioreactors

(iv) Disarmed pathogen vectors

23.

Q24. Which one of the following is a case of wrong matching?

(i) Vector DNA – Site for t-RNA synthesis

(ii) Somatic hybridisation – Fusion of two diverse cells

(iii) Callus – Unorganised mass of cell produced in tissue culture

(iv) Micropropagation – In vitro production of plants in large numbers

24.

Q25. DNA obtained for DNA finger printing:

(i) W.B.C.

(ii) Hair root cells

(iii) Body fluids

(iv) None of these

25.

Q26. Which one of the following is not a correct match ?

(i) Tumour inducing – Ti plasmid

(ii) DNA probe – Identifies the desired DNA

(iii) PCR – DNA staining

(iv) Agarose – Seaweeds

26.

Q27. Electroporation facilitates introduction of foreign DNA into the target organism by

(i) Changing the porosity of the cell wall

(ii) Changing the electric potential of the cell wall

(iii) Lysis of the cell wall

(iv) Active transport across the cell wall

27.

Q28. The transfer of genetic material from one bacterium to another through the mediation of a vector-like virus is termed as:

(i) Transduction

(ii) Conjugation

(iii) Transformation

(iv) Translation.

28.

Q29. In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

(i) Charge only

(ii) Size only

(iii) Charge to size ratio

(iv) All of the above.

29.

Q30. Restriction endonuclease cuts:

(i) One stand of DNA at specific site

(ii) Both strands of DNA

(iii) Both strands of DNA at any site

(iv) Single strand of RNA

30.

Q31. A device in which large volume of living cells are cultured in order to get a specific product is called

(i) PCR

(ii) agitator

(iii) bioreactor

(iv) assimilator.

31.

Q32. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:

(i) Silver or Platinum

(ii) Gold or Tungsten

(iii) Silicon or Platinum

(iv) Platinum or Zinc

32.

Q33. Golden rice is a promising transgenic crop. When released for cultivation, it will help in :

(i) High lysin content

(ii) Pest resistance

(iii) High protien content

(iv) High vitamin A content

33.

Q34. An enzyme catalysing the removal of nucleotides from the ends of DNA is

(i) endonuclease

(ii) exonuclease

(iii) DNA ligase

(iv) Hind II.

34.

Q35. Restriction enzymes belongs to a larger class of enzymes called?

(i) Nucleases

(ii) Glcucatase

(iii) Chitinase

(iv) Protease

35.

Q36. While isolating DNA from bacteria, which of the following enzymes is not used?

(i) Lyozyme

(ii) Ribonuclease

(iii) Deoxyribonuclease

(iv) Protease.

36.

Q37. Triticale, first man made cereal crop, has been obtained by crossing wheat with:

(i) Rye

(ii) Pearl millet

(iii) Sugarcane

(iv) Barley

37.

Q38. Who is the father of genetic engineering ?

(i) Steward Linn

(ii) Stanley Cohen

(iii) Paul Berg

(iv) Kary Mullis

38.

Q39. Insertional inactivation helps in

(i) X-gal breakdown

(ii) Identification of recombinant clones

(iii) Ampicillin resistance

(iv) Functioning of B-galactosidase

39.

Q 40. Important objective of biotechnology in agriculture section is

(a) To produce pest resistant varieties of plants

(b) To increase the nitrogen content

(c) To decrease the seed number

(d) To increase the plant weight

40.

Q 41. The two vitamins manufactured biotechnologically are

(a) Vitamin B12 and Vitamin B6

(b) Vitamin B12 and Vitamin B2

(c) Vitamin B6 and Vitamin B2

(d) Vitamin B12 and Vitamin B9

41.

Q 42. Some pathogenic bacteria develop resistance to antibiotics by

(a) Modifying their cell walls

(b) Developing such enzymes which modify antibiotics

(c) Alter the antibiotics target due to spontaneous mutation

(d) All the above.

42.

Q 43. What was the world’s first ever mammal to be successfully cloned from an adult cell?

(a) Sheep

(b) Monkey

(c) Cow

(d) Calf

43.

Q 44. Micro-organisms can be grown in the bioreactors by

(a) Support growth system

(b) Agitated growth system

(c) Suspended .growth system

(d) Both (a) and (c)

44.

Q 45. Name the drug used in cancer treatment produced by using biotechnology

(a) Terramycin

(b) HGH (c) Insulin

(c) TSH

d) Enterferon

45.

Q 46. During ‘gene cloning’ which is called as ‘gene taxi’?

(a) Vaccine

(b) Plasmid

(c) Bacterium

(d) Protozoa

46.

Q 47. Hybridoma technology has been successfully used in

(a) production of somatic hybrids

(b) synthesis of monoclonal antibodies

(c) synthesis of haemoglobin

(d) production of alcohol in bulk

47.

Q 48. Introduction of food plants developed by genetic engineering is not desirable because

(a) These products are less tasty as compared to the already existing products

(b) The method is costly

(c) Economy of developing countries may suffer.

(d) There is danger of entry of viruses and toxins with introduced crop

48.

Q 49. Hybridoma cells are

(a) Nervous cells of frog

(b) Hybrid cells resulting from myeloma cells

(c) Only cells having oncogenes

(d) Product of spore formation in bacteria

49.

Q 50. The new strain of bacteria produced by biotechnology in alcohol industry is

(a) Escherichia coli

(b) Saccharomyces cerevisiae

(c) Bacillus sabtilis

(d) Pseudomonas putida

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